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Free 25-Hydroxyvitamin D Concentrations in Cystic Fibrosis.


ABSTRACT: BACKGROUND:Vitamin D deficiency is common in cystic fibrosis (CF), but there is no previous data on free 25-hydroxyvitamin D (25[OH]D) in CF or in relation to healthy individuals. METHODS:We assessed total serum 25(OH)D concentration by chemiluminescence and serum free 25(OH)D concentration by both direct measurement (ELISA) and calculation, using serum albumin and vitamin D binding protein (VDBP) levels in 80 subjects (28 healthy adults, 25 clinically stable adults and children with CF and 27 adults experiencing a CF exacerbation). RESULTS:Serum albumin and VDBP concentrations were lower in CF compared with healthy controls. Total serum 25(OH)D concentrations were positively correlated with both calculated and measured free 25(OH)D (P < 0.001 for both). Calculated and directly measured serum free 25(OH)D levels were positively correlated (P < 0.001). CONCLUSIONS:Serum levels of directly measured free 25(OH)D positively correlated with total 25(OH)D, suggesting that achieving sufficient total serum 25(OH)D may result in adequate free 25(OH)D levels in CF.

SUBMITTER: Lee MJ 

PROVIDER: S-EPMC4629503 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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Free 25-Hydroxyvitamin D Concentrations in Cystic Fibrosis.

Lee Moon Jeong MJ   Kearns Malcolm D MD   Smith Ellen M EM   Hao Li L   Ziegler Thomas R TR   Alvarez Jessica A JA   Tangpricha Vin V  

The American journal of the medical sciences 20151101 5


<h4>Background</h4>Vitamin D deficiency is common in cystic fibrosis (CF), but there is no previous data on free 25-hydroxyvitamin D (25[OH]D) in CF or in relation to healthy individuals.<h4>Methods</h4>We assessed total serum 25(OH)D concentration by chemiluminescence and serum free 25(OH)D concentration by both direct measurement (ELISA) and calculation, using serum albumin and vitamin D binding protein (VDBP) levels in 80 subjects (28 healthy adults, 25 clinically stable adults and children w  ...[more]

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