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Recent structural studies on Dom34/aPelota and Hbs1/aEF1?: important factors for solving general problems of ribosomal stall in translation.

ABSTRACT: In the translation process, translating ribosomes usually move on an mRNA until they reach the stop codon. However, when ribosomes translate an aberrant mRNA, they stall. Then, ribosomes are rescued from the aberrant mRNA, and the aberrant mRNA is subsequently degraded. In eukaryotes, Pelota (Dom34 in yeast) and Hbs1 are responsible for solving general problems of ribosomal stall in translation. In archaea, aPelota and aEF1?, homologous to Pelota and Hbs1, respectively, are considered to be involved in that process. In recent years, great progress has been made in determining structures of Dom34/aPelota and Hbs1/aEF1?. In this review, we focus on the functional roles of Dom34/aPelota and Hbs1/aEF1? in ribosome rescue, based on recent structural studies of them. We will also present questions to be answered by future work.

SUBMITTER: Kobayashi K

PROVIDER: S-EPMC4629679 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Recent structural studies on Dom34/aPelota and Hbs1/aEF1α: important factors for solving general problems of ribosomal stall in translation.

Kobayashi Kan K Ishitani Ryuichiro R Nureki Osamu O

Biophysics (Nagoya-shi, Japan) 20130907

In the translation process, translating ribosomes usually move on an mRNA until they reach the stop codon. However, when ribosomes translate an aberrant mRNA, they stall. Then, ribosomes are rescued from the aberrant mRNA, and the aberrant mRNA is subsequently degraded. In eukaryotes, Pelota (Dom34 in yeast) and Hbs1 are responsible for solving general problems of ribosomal stall in translation. In archaea, aPelota and aEF1α, homologous to Pelota and Hbs1, respectively, are considered to be invo ...[more]

PMID: 27493551

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