Subtractive assembly for comparative metagenomics, and its application to type 2 diabetes metagenomes.
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ABSTRACT:
SUBMITTER: Wang M
PROVIDER: S-EPMC4630832 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Publications
Subtractive assembly for comparative metagenomics, and its application to type 2 diabetes metagenomes.
Wang Mingjie M Doak Thomas G TG Ye Yuzhen Y
Genome biology 20151102
Comparative metagenomics remains challenging due to the size and complexity of metagenomic datasets. Here we introduce subtractive assembly, a de novo assembly approach for comparative metagenomics that directly assembles only the differential reads that distinguish between two groups of metagenomes. Using simulated datasets, we show it improves both the efficiency of the assembly and the assembly quality of the differential genomes and genes. Further, its application to type 2 diabetes (T2D) me ...[more]
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