Ontology highlight
ABSTRACT:
SUBMITTER: Grossman I
PROVIDER: S-EPMC4634331 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Nature communications 20151015
The ability to query enzyme molecules individually is transforming our view of catalytic mechanisms. Quiescin sulfhydryl oxidase (QSOX) is a multidomain catalyst of disulfide-bond formation that relays electrons from substrate cysteines through two redox-active sites to molecular oxygen. The chemical steps in electron transfer have been delineated, but the conformational changes accompanying these steps are poorly characterized. Here we use single-molecule Förster resonance energy transfer (smFR ...[more]