Ontology highlight
ABSTRACT:
SUBMITTER: Fan B
PROVIDER: S-EPMC4638032 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Fan Baochang B Sutandy F X Reymond FX Syu Guan-Da GD Middleton Stefani S Yi Guanghui G Lu Kuan-Yi KY Chen Chien-Sheng CS Kao C Cheng CC
Molecular & cellular proteomics : MCP 20150901 11
Heterogeneous ribonucleoprotein K (hnRNP K) binds to the 5' untranslated region of the hepatitis C virus (HCV) and is required for HCV RNA replication. The hnRNP K binding site on HCV RNA overlaps with the sequence recognized by the liver-specific microRNA, miR-122. A proteome chip containing ∼17,000 unique human proteins probed with miR-122 identified hnRNP K as one of the strong binding proteins. In vitro kinetic study showed hnRNP K binds miR-122 with a nanomolar dissociation constant, in whi ...[more]