Dataset Information

Construction, complete sequence, and annotation of a BAC contig covering the silkworm chorion locus.

ABSTRACT: The silkmoth chorion was studied extensively by F.C. Kafatos' group for almost 40 years. However, the complete structure of the chorion locus was not obtained in the genome sequence of Bombyx mori published in 2008 due to repetitive sequences, resulting in gaps and an incomplete view of the locus. To obtain the complete sequence of the chorion locus, expressed sequence tags (ESTs) derived from follicular epithelium cells were used as probes to screen a bacterial artificial chromosome (BAC) library. Seven BACs were selected to construct a contig which covered the whole chorion locus. By Sanger sequencing, we successfully obtained complete sequences of the chorion locus spanning 871,711 base pairs on chromosome 2, where we annotated 127 chorion genes. The dataset reported here will recruit more researchers to revisit one of the oldest model systems which has been used to study developmentally regulated gene expression. It also provides insights into egg development and fertilization mechanisms and is relevant to applications related to improvements in breeding procedures and transgenesis.

SUBMITTER: Chen Z

PROVIDER: S-EPMC4640134 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Construction, complete sequence, and annotation of a BAC contig covering the silkworm chorion locus.

Chen Zhiwei Z Nohata Junko J Guo Huizhen H Li Shenglong S Liu Jianqiu J Guo Youbing Y Yamamoto Kimiko K Kadono-Okuda Keiko K Liu Chun C Arunkumar Kallare P KP Nagaraju Javaregowda J Zhang Yan Y Liu Shiping S Labropoulou Vassiliki V Swevers Luc L Tsitoura Panagiota P Iatrou Kostas K Gopinathan Karumathil P KP Goldsmith Marian R MR Xia Qingyou Q Mita Kazuei K

Scientific data 20151110

The silkmoth chorion was studied extensively by F.C. Kafatos' group for almost 40 years. However, the complete structure of the chorion locus was not obtained in the genome sequence of Bombyx mori published in 2008 due to repetitive sequences, resulting in gaps and an incomplete view of the locus. To obtain the complete sequence of the chorion locus, expressed sequence tags (ESTs) derived from follicular epithelium cells were used as probes to screen a bacterial artificial chromosome (BAC) libra ...[more]

PMID: 26594380

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Dataset Information

Construction, complete sequence, and annotation of a BAC contig covering the silkworm chorion locus.

Publications

Construction, complete sequence, and annotation of a BAC contig covering the silkworm chorion locus.

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