Ontology highlight
ABSTRACT:
SUBMITTER: Mageean CJ
PROVIDER: S-EPMC4641634 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Mageean Craig J CJ Griffiths John R JR Smith Duncan L DL Clague Michael J MJ Prior Ian A IA
PloS one 20151111 11
Ras proteins are important signalling hubs situated near the top of networks controlling cell proliferation, differentiation and survival. Three almost identical isoforms, HRAS, KRAS and NRAS, are ubiquitously expressed yet have differing biological and oncogenic properties. In order to help understand the relative biological contributions of each isoform we have optimised a quantitative proteomics method for accurately measuring Ras isoform protein copy number per cell. The use of isotopic prot ...[more]