Ontology highlight
ABSTRACT:
SUBMITTER: Wang B
PROVIDER: S-EPMC4644663 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Wang Bo B Yu Jianping J Zhang Weiwen W Meldrum Deirdre R DR
Applied and environmental microbiology 20151009 24
Restriction digestion of foreign DNA is one of the key biological barriers against genetic transformation in microorganisms. To establish a high-efficiency transformation protocol in the model cyanobacterium, Synechocystis sp. strain PCC 6803 (Synechocystis 6803), we investigated the effects of premethylation of foreign DNA on the integrative transformation of this strain. In this study, two type II methyltransferase-encoding genes, i.e., sll0729 (gene M) and slr0214 (gene C), were cloned from t ...[more]