Ontology highlight
ABSTRACT:
SUBMITTER: Stefanetti G
PROVIDER: S-EPMC4648054 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Stefanetti Giuseppe G Hu Qi-Ying QY Usera Aimee A Robinson Zack Z Allan Martin M Singh Alok A Imase Hidetomo H Cobb Jennifer J Zhai Huili H Quinn Douglas D Lei Ming M Saul Allan A Adamo Roberto R MacLennan Calman A CA Micoli Francesca F
Angewandte Chemie (International ed. in English) 20150909 45
A series of glycoconjugates with defined connectivity were synthesized to investigate the impact of coupling Salmonella typhimurium O-antigen to different amino acids of CRM197 protein carrier. In particular, two novel methods for site-selective glycan conjugation were developed to obtain conjugates with single attachment site on the protein, based on chemical modification of a disulfide bond and pH-controlled transglutaminase-catalyzed modification of lysine, respectively. Importantly, conjugat ...[more]