Ontology highlight
ABSTRACT:
SUBMITTER: Arbab M
PROVIDER: S-EPMC4649464 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Arbab Mandana M Srinivasan Sharanya S Hashimoto Tatsunori T Geijsen Niels N Sherwood Richard I RI
Stem cell reports 20151029 5
We present self-cloning CRISPR/Cas9 (scCRISPR), a technology that allows for CRISPR/Cas9-mediated genomic mutation and site-specific knockin transgene creation within several hours by circumventing the need to clone a site-specific single-guide RNA (sgRNA) or knockin homology construct for each target locus. We introduce a self-cleaving palindromic sgRNA plasmid and a short double-stranded DNA sequence encoding the desired locus-specific sgRNA into target cells, allowing them to produce a locus- ...[more]