Dataset Information

The Transgenic RNAi Project at Harvard Medical School: Resources and Validation.

ABSTRACT: To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT-qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection, which is currently composed of 11,491 lines and covering 71% of Drosophila genes. Data on the characterization of the lines either by RT-qPCR or phenotype is available on a dedicated website, the RNAi Stock Validation and Phenotypes Project (RSVP, http://www.flyrnai.org/RSVP.html), and stocks are available from three stock centers, the Bloomington Drosophila Stock Center (United States), National Institute of Genetics (Japan), and TsingHua Fly Center (China).

SUBMITTER: Perkins LA

PROVIDER: S-EPMC4649654 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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The Transgenic RNAi Project at Harvard Medical School: Resources and Validation.

Perkins Lizabeth A LA Holderbaum Laura L Tao Rong R Hu Yanhui Y Sopko Richelle R McCall Kim K Yang-Zhou Donghui D Flockhart Ian I Binari Richard R Shim Hye-Seok HS Miller Audrey A Housden Amy A Foos Marianna M Randkelv Sakara S Kelley Colleen C Namgyal Pema P Villalta Christians C Liu Lu-Ping LP Jiang Xia X Huan-Huan Qiao Q Wang Xia X Fujiyama Asao A Toyoda Atsushi A Ayers Kathleen K Blum Allison A Czech Benjamin B Neumuller Ralph R Yan Dong D Cavallaro Amanda A Hibbard Karen K Hall Don D Cooley Lynn L Hannon Gregory J GJ Lehmann Ruth R Parks Annette A Mohr Stephanie E SE Ueda Ryu R Kondo Shu S Ni Jian-Quan JQ Perrimon Norbert N

Genetics 20150828 3

To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT-qPCR and phenotypic analyses, and developing tools ...[more]

PMID: 26320097

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Dataset Information

The Transgenic RNAi Project at Harvard Medical School: Resources and Validation.

Publications

The Transgenic RNAi Project at Harvard Medical School: Resources and Validation.

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