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Identification of General and Heart-Specific miRNAs in Sheep (Ovis aries).

ABSTRACT: MicroRNAs (miRNAs or miRs) are small regulatory RNAs crucial for modulation of signaling pathways in multiple organs. While the link between miRNAs and heart disease has grown more readily apparent over the past three years, these data are primarily limited to small animal models or cell-based systems. Here, we performed a high-throughput RNA sequencing (RNAseq) analysis of left ventricle and other tissue from a pre-clinical ovine model. We identified 172 novel miRNA precursors encoding a total of 264 mature miRNAs. Notably, 84 precursors were detected in both the left ventricle and other tissues. However, 10 precursors, encoding 11 mature sequences, were specific to the left ventricle. Moreover, the total 168 novel miRNA precursors included 22 non-conserved ovine-specific sequences. Our data identify and characterize novel miRNAs in the left ventricle of sheep, providing fundamental new information for our understanding of protein regulation in heart and other tissues.

SUBMITTER: Lagana A

PROVIDER: S-EPMC4657999 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Identification of General and Heart-Specific miRNAs in Sheep (Ovis aries).

Laganà Alessandro A Veneziano Dario D Spata Tyler T Tang Richard R Zhu Hua H Mohler Peter J PJ Kilic Ahmet A

PloS one 20151124 11

MicroRNAs (miRNAs or miRs) are small regulatory RNAs crucial for modulation of signaling pathways in multiple organs. While the link between miRNAs and heart disease has grown more readily apparent over the past three years, these data are primarily limited to small animal models or cell-based systems. Here, we performed a high-throughput RNA sequencing (RNAseq) analysis of left ventricle and other tissue from a pre-clinical ovine model. We identified 172 novel miRNA precursors encoding a total ...[more]

PMID: 26599010

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