Unknown

Dataset Information

0

Langerhans cells from women with cervical precancerous lesions become functionally responsive against human papillomavirus after activation with stabilized Poly-I:C.


ABSTRACT: Human papillomavirus (HPV)-mediated suppression of Langerhans cell (LC) function can lead to persistent infection and development of cervical intraepithelial neoplasia (CIN). Women with HPV-induced high-grade CIN2/3 have not mounted an effective immune response against HPV, yet it is unknown if LC-mediated T cell activation from such women is functionally impaired against HPV. We investigated the functional activation of in vitro generated LC and their ability to induce HPV16-specific T cells from CIN2/3 patients after exposure to HPV16 followed by treatment with stabilized Poly-I:C (s-Poly-I:C). LC from patients exposed to HPV16 demonstrated a lack of costimulatory molecule expression, inflammatory cytokine secretion, and chemokine-directed migration. Conversely, s-Poly-I:C caused significant phenotypic and functional activation of HPV16-exposed LC, which resulted in de novo generation of HPV16-specific CD8(+) T cells. Our results highlight that LC of women with a history of persistent HPV infection can present HPV antigens and are capable of inducing an adaptive T cell immune response when given the proper stimulus, suggesting that s-Poly-I:C compounds may be attractive immunomodulators for LC-mediated clearance of persistent HPV infection.

SUBMITTER: Da Silva DM 

PROVIDER: S-EPMC4658296 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Langerhans cells from women with cervical precancerous lesions become functionally responsive against human papillomavirus after activation with stabilized Poly-I:C.

Da Silva Diane M DM   Woodham Andrew W AW   Skeate Joseph G JG   Rijkee Laurie K LK   Taylor Julia R JR   Brand Heike E HE   Muderspach Laila I LI   Roman Lynda D LD   Yessaian Annie A AA   Pham Huyen Q HQ   Matsuo Koji K   Lin Yvonne G YG   McKee Greg M GM   Salazar Andres M AM   Kast W Martin WM  

Clinical immunology (Orlando, Fla.) 20150908 2


Human papillomavirus (HPV)-mediated suppression of Langerhans cell (LC) function can lead to persistent infection and development of cervical intraepithelial neoplasia (CIN). Women with HPV-induced high-grade CIN2/3 have not mounted an effective immune response against HPV, yet it is unknown if LC-mediated T cell activation from such women is functionally impaired against HPV. We investigated the functional activation of in vitro generated LC and their ability to induce HPV16-specific T cells fr  ...[more]

Similar Datasets

| S-EPMC4671084 | biostudies-literature
2023-05-07 | GSE167858 | GEO
| S-EPMC3986944 | biostudies-literature
| S-EPMC7607420 | biostudies-literature
| S-EPMC6765587 | biostudies-literature
| S-EPMC8018444 | biostudies-literature
| S-EPMC9301573 | biostudies-literature
| PRJNA705230 | ENA
| S-EPMC5883188 | biostudies-literature
| S-EPMC3024957 | biostudies-literature