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Data for proteomic analysis of ATP-binding proteins and kinase inhibitor target proteins using an ATP probe.

ABSTRACT: Interactions between ATP and ATP-binding proteins (ATPome) are common and are required for most cellular processes. Thus, it is clearly important to identify and quantify these interactions for understanding basic cellular mechanisms and the pathogenesis of various diseases. We used an ATP competition assay (competition between ATP and acyl-ATP probes) that enabled us to distinguish specific ATP-binding proteins from non-specific proteins (Adachi et al., 2014) [1]. As a result, we identified 539 proteins, including 178 novel ATP-binding protein candidates. We also established an ATPome selectivity profiling method for kinase inhibitors using our cataloged ATPome list. Normally only kinome selectivity is profiled in selectivity profiling of kinase inhibitors. In this data, we expand the profiled targets from the kinome to the ATPome through performance of ATPome selectivity profiling and obtained target profiles of staurosporine and (S)-crizotinib. The data accompanying the manuscript on this approach (Adachi et al., 2014) [1] have been deposited to the ProteomeXchange with identifier PXD001200.

SUBMITTER: Adachi J 

PROVIDER: S-EPMC4659801 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Data for proteomic analysis of ATP-binding proteins and kinase inhibitor target proteins using an ATP probe.

Adachi Jun J   Kishida Marina M   Watanabe Shio S   Hashimoto Yuuki Y   Fukamizu Kazuna K   Tomonaga Takeshi T  

Data in brief 20151026

Interactions between ATP and ATP-binding proteins (ATPome) are common and are required for most cellular processes. Thus, it is clearly important to identify and quantify these interactions for understanding basic cellular mechanisms and the pathogenesis of various diseases. We used an ATP competition assay (competition between ATP and acyl-ATP probes) that enabled us to distinguish specific ATP-binding proteins from non-specific proteins (Adachi et al., 2014) [1]. As a result, we identified 539  ...[more]