Ontology highlight
ABSTRACT:
SUBMITTER: Schmidt TL
PROVIDER: S-EPMC4660042 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Schmidt Thorsten L TL Beliveau Brian J BJ Uca Yavuz O YO Theilmann Mark M Da Cruz Felipe F Wu Chao-Ting CT Shih William M WM
Nature communications 20151116
Synthetic oligonucleotides are the main cost factor for studies in DNA nanotechnology, genetics and synthetic biology, which all require thousands of these at high quality. Inexpensive chip-synthesized oligonucleotide libraries can contain hundreds of thousands of distinct sequences, however only at sub-femtomole quantities per strand. Here we present a selective oligonucleotide amplification method, based on three rounds of rolling-circle amplification, that produces nanomole amounts of single- ...[more]