Identification of low abundance microbiome in clinical samples using whole genome sequencing.
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ABSTRACT:
SUBMITTER: Zhang C
PROVIDER: S-EPMC4661937 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Publications
Identification of low abundance microbiome in clinical samples using whole genome sequencing.
Zhang Chao C Cleveland Kyle K Schnoll-Sussman Felice F McClure Bridget B Bigg Michelle M Thakkar Prashant P Schultz Nikolaus N Shah Manish A MA Betel Doron D
Genome biology 20151127
Identifying the microbiome composition from primary tissues directly affords an opportunity to study the causative relationships between the host microbiome and disease. However, this is challenging due the low abundance of microbial DNA relative to the host. We present a systematic evaluation of microbiome profiling directly from endoscopic biopsies by whole genome sequencing. We compared our methods with other approaches on datasets with previously identified microbial composition. We applied ...[more]
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