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Tandem Domains with Tuned Interactions Are a Powerful Biological Design Principle.

ABSTRACT: Allosteric effects of mutations, ligand binding, or post-translational modifications on protein function occur through changes to the protein's shape, or conformation. In a cell, there are many copies of the same protein, all experiencing these perturbations in a dynamic fashion and fluctuating through different conformations and activity states. According to the "conformational selection and population shift" theory, ligand binding selects a particular conformation. This perturbs the ensemble and induces a population shift. In a new PLOS Biology paper, Melacini and colleagues describe a novel model of protein regulation, the "Double-Conformational Selection Model", which demonstrates how two tandem ligand-binding domains interact to regulate protein function. Here we explain how tandem domains with tuned interactions-but not single domains-can provide a blueprint for sensitive activation sensors within a narrow window of ligand concentration, thereby promoting signaling control.

SUBMITTER: Nussinov R

PROVIDER: S-EPMC4664463 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Tandem Domains with Tuned Interactions Are a Powerful Biological Design Principle.

Nussinov Ruth R Tsai Chung-Jung CJ

PLoS biology 20151130 11

Allosteric effects of mutations, ligand binding, or post-translational modifications on protein function occur through changes to the protein's shape, or conformation. In a cell, there are many copies of the same protein, all experiencing these perturbations in a dynamic fashion and fluctuating through different conformations and activity states. According to the "conformational selection and population shift" theory, ligand binding selects a particular conformation. This perturbs the ensemble a ...[more]

PMID: 26618518

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