Mammalian Mss51 is a skeletal muscle-specific gene modulating cellular metabolism.

ABSTRACT:

Background

The transforming growth factor β (TGF-β) signaling pathways modulate skeletal muscle growth, regeneration, and cellular metabolism. Several recent gene expression studies have shown that inhibition of myostatin and TGF-β1 signaling consistently leads to a significant reduction in expression of Mss51, also named Zmynd17. The function of mammalian Mss51 is unknown although a putative homolog in yeast is a mitochondrial translational activator.

Objective

The objective of this work was to characterize mammalian Mss51.

Methods

Quantitative RT-PCR and immunoblot of subcellular fractionation were used to determine expression patterns and localization of Mss51. The CRISPR/Cas9 system was used to reduce expression of Mss51 in C2C12 myoblasts and the function of Mss5

SUBMITTER: Moyer AL 

PROVIDER: S-EPMC4664537 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature