Transcriptomic profiling of human embryonic stem cells upon cell cycle manipulation during pluripotent state dissolution.

ABSTRACT: While distinct cell cycle structures have been known to correlate with pluripotent or differentiated cell states [1], there is no evidence on how the cell cycle machinery directly contributes to human embryonic stem cell (hESC) pluripotency. We established a determinant role of cell cycle machineries on the pluripotent state by demonstrating that the specific perturbation of the S and G2 phases can prevent pluripotent state dissolution (PSD) [2]. Active mechanisms in these phases, such as the DNA damage checkpoint and Cyclin B1, promote the pluripotent state [2]. To understand the mechanisms behind the effect on PSD by these pathways in hESCs, we performed comprehensive gene expression analysis by time-course microarray experiments. From these datasets, we observed expression changes in ge

SUBMITTER: Gonzales KA 

PROVIDER: S-EPMC4664715 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature