Dataset Information

Microarray analyses during early stage of the tomato/Alternaria solani interaction.

ABSTRACT: Tomato early blight is an important threat and it has capacity to reduce the production in all major tomato producing areas. Molecular mechanism underlying the resistance against this is not well known. Therefore we studied this system to search the possible mechanism of resistance, which includes pathogenesis related protein, and pathways and transcription factors, which are responsible for resistance against this pathogen using affymetrix gene chip for tomato. Their differential expressions have enhanced the biochemical and other related products, which have, direct or indirect role in stopping the penetration of mycelia in the host plant.

SUBMITTER: Upadhyay P

PROVIDER: S-EPMC4664743 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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Microarray analyses during early stage of the tomato/Alternaria solani interaction.

Upadhyay Priti P Rai Ashutosh A Kumar Rajesh R Singh Major M Sinha Brajesh B

Genomics data 20150909

Tomato early blight is an important threat and it has capacity to reduce the production in all major tomato producing areas. Molecular mechanism underlying the resistance against this is not well known. Therefore we studied this system to search the possible mechanism of resistance, which includes pathogenesis related protein, and pathways and transcription factors, which are responsible for resistance against this pathogen using affymetrix gene chip for tomato. Their differential expressions ha ...[more]

PMID: 26697364

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Project description:Tomato (Solanum lycopersicum) is among the most important commercial horticultural crops worldwide. The crop quality and production is largely hampered due to the fungal pathogen Alternaria solani causing necrotrophic foliage early blight disease. Crop plants usually respond to the biotic challenges with altered metabolic composition and physiological perturbations. We have deciphered altered metabolite composition, modulated metabolic pathways and identified metabolite biomarkers in A. solani-challenged susceptible tomato variety Kashi Aman using Liquid Chromatography-Mass Spectrometry (LC-MS) based metabolomics. Alteration in the metabolite feature composition of pathogen-challenged (m/z 9405) and non-challenged (m/z 9667) plant leaves including 8487 infection-exclusive and 8742 non-infection exclusive features was observed. Functional annotation revealed putatively annotated metabolites and pathway mapping indicated their enrichment in metabolic pathways, biosynthesis of secondary metabolites, ubiquinone and terpenoid-quinones, brassinosteroids, steroids, terpenoids, phenylpropanoids, carotenoids, oxy/sphingolipids and metabolism of biotin and porphyrin. PCA, multivariate PLS-DA and OPLS-DA analysis showed sample discrimination. Significantly up regulated 481 and down regulated 548 metabolite features were identified based on the fold change (threshold ≥ 2.0). OPLS-DA model based on variable importance in projection (VIP scores) and FC threshold (> 2.0) revealed 41 up regulated discriminant metabolite features annotated as sphingosine, fecosterol, melatonin, serotonin, glucose 6-phosphate, zeatin, dihydrozeatin and zeatin-β-D-glucoside. Similarly, 23 down regulated discriminant metabolites included histidinol, 4-aminobutyraldehyde, propanoate, tyramine and linalool. Melatonin and serotonin in the leaves were the two indoleamines being reported for the first time in tomato in response to the early blight pathogen. Receiver operating characteristic (ROC)-based biomarker analysis identified apigenin-7-glucoside, uridine, adenosyl-homocysteine, cGMP, tyrosine, pantothenic acid, riboflavin (as up regulated) and adenosine, homocyctine and azmaline (as down regulated) biomarkers. These results could aid in the development of metabolite-quantitative trait loci (mQTL). Furthermore, stress-induced biosynthetic pathways may be the potential targets for modifications through breeding programs or genetic engineering for improving crop performance in the fields.

Dataset Information

Microarray analyses during early stage of the tomato/Alternaria solani interaction.

Publications

Microarray analyses during early stage of the tomato/Alternaria solani interaction.

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