Cas9 gRNA engineering for genome editing, activation and repression.
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ABSTRACT:
SUBMITTER: Kiani S
PROVIDER: S-EPMC4666719 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Publications
Cas9 gRNA engineering for genome editing, activation and repression.
Kiani Samira S Chavez Alejandro A Tuttle Marcelle M Hall Richard N RN Chari Raj R Ter-Ovanesyan Dmitry D Qian Jason J Pruitt Benjamin W BW Beal Jacob J Vora Suhani S Buchthal Joanna J Kowal Emma J K EJ Ebrahimkhani Mohammad R MR Collins James J JJ Weiss Ron R Church George G
Nature methods 20150907 11
We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein. ...[more]
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