Ontology highlight
ABSTRACT:
SUBMITTER: Thakore PI
PROVIDER: S-EPMC4666778 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Thakore Pratiksha I PI D'Ippolito Anthony M AM Song Lingyun L Safi Alexias A Shivakumar Nishkala K NK Kabadi Ami M AM Reddy Timothy E TE Crawford Gregory E GE Gersbach Charles A CA
Nature methods 20151026 12
Epigenome editing with the CRISPR (clustered, regularly interspaced, short palindromic repeats)-Cas9 platform is a promising technology for modulating gene expression to direct cell phenotype and to dissect the causal epigenetic mechanisms of gene regulation. Fusions of nuclease-inactive dCas9 to the Krüppel-associated box (KRAB) repressor (dCas9-KRAB) can silence target gene expression, but the genome-wide specificity and the extent of heterochromatin formation catalyzed by dCas9-KRAB are not k ...[more]