Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy.
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ABSTRACT:
SUBMITTER: Scarcelli G
PROVIDER: S-EPMC4666809 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Publications
Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy.
Scarcelli Giuliano G Polacheck William J WJ Nia Hadi T HT Patel Kripa K Grodzinsky Alan J AJ Kamm Roger D RD Yun Seok Hyun SH
Nature methods 20151005 12
Current measurements of the biomechanical properties of cells require physical contact with cells or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable of measuring an intracellular longitudinal modulus with optical resolution. The 3D Brillouin maps we obtained of cells in 2D and 3D microenvironments revealed mechanical changes due to cytoskeletal modulation and cell-volume regulation. ...[more]
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