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Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera.

ABSTRACT: Lactobacilli are a diverse group of species that occupy diverse nutrient-rich niches associated with humans, animals, plants and food. They are used widely in biotechnology and food preservation, and are being explored as therapeutics. Exploiting lactobacilli has been complicated by metabolic diversity, unclear species identity and uncertain relationships between them and other commercially important lactic acid bacteria. The capacity for biotransformations catalysed by lactobacilli is an untapped biotechnology resource. Here we report the genome sequences of 213 Lactobacillus strains and associated genera, and their encoded genetic catalogue for modifying carbohydrates and proteins. In addition, we describe broad and diverse presence of novel CRISPR-Cas immune systems in lactobacilli that may be exploited for genome editing. We rationalize the phylogenomic distribution of host interaction factors and bacteriocins that affect their natural and industrial environments, and mechanisms to withstand stress during technological processes. We present a robust phylogenomic framework of existing species and for classifying new species.

SUBMITTER: Sun Z

PROVIDER: S-EPMC4667430 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

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Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera.

Sun Zhihong Z Harris Hugh M B HM McCann Angela A Guo Chenyi C Argimón Silvia S Zhang Wenyi W Yang Xianwei X Jeffery Ian B IB Cooney Jakki C JC Kagawa Todd F TF Liu Wenjun W Song Yuqin Y Salvetti Elisa E Wrobel Agnieszka A Rasinkangas Pia P Parkhill Julian J Rea Mary C MC O'Sullivan Orla O Ritari Jarmo J Douillard François P FP Paul Ross R R Yang Ruifu R Briner Alexandra E AE Felis Giovanna E GE de Vos Willem M WM Barrangou Rodolphe R Klaenhammer Todd R TR Caufield Page W PW Cui Yujun Y Zhang Heping H O'Toole Paul W PW

Nature communications 20150929

Lactobacilli are a diverse group of species that occupy diverse nutrient-rich niches associated with humans, animals, plants and food. They are used widely in biotechnology and food preservation, and are being explored as therapeutics. Exploiting lactobacilli has been complicated by metabolic diversity, unclear species identity and uncertain relationships between them and other commercially important lactic acid bacteria. The capacity for biotransformations catalysed by lactobacilli is an untapp ...[more]

PMID: 26415554

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Dataset Information

Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera.

Publications

Expanding the biotechnology potential of lactobacilli through comparative genomics of 213 strains and associated genera.

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