Ontology highlight
ABSTRACT:
SUBMITTER: Fried SD
PROVIDER: S-EPMC4668018 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20141201 6216
Enzymes use protein architecture to impose specific electrostatic fields onto their bound substrates, but the magnitude and catalytic effect of these electric fields have proven difficult to quantify with standard experimental approaches. Using vibrational Stark effect spectroscopy, we found that the active site of the enzyme ketosteroid isomerase (KSI) exerts an extremely large electric field onto the C=O chemical bond that undergoes a charge rearrangement in KSI's rate-determining step. Moreov ...[more]