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In-Source Decay Characterization of Isoaspartate and ?-Peptides.


ABSTRACT: Deamidation and the subsequent formation of isoaspartic acid (isoAsp) are common modifications of asparagine (Asn) residues in proteins. Differentiation of isoAsp and Asp residues is a challenging task owing to their similar chemical properties and identical molecular mass. Recent studies showed that they can be differentiated using electron capture dissociation (ECD) which generates diagnostic fragments c'+57 and z•-57 specific to the isoAsp residue. However, the ECD approach is only applicable towards multiply charged precursor ions and generally does not work for ?-amino acids other than isoAsp. In this study, the potential of in-source decay (ISD) in characterization of isoAsp and other ?-amino acids was explored. For isoAsp-containing peptides, ISD with a conventional hydrogen-donating matrix produced ECD-like, c'+57 and z•-57 diagnostic ions, even for singly charged precursor ions. For other ?-amino acids, a hydrogen-accepting matrix was used to induce formation of site-specific a-14 ions from a synthetic ?-analogue of substance P. These results indicated that ISD can be broadly applied for ?-peptide characterization.

SUBMITTER: Yu X 

PROVIDER: S-EPMC4669973 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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In-Source Decay Characterization of Isoaspartate and β-Peptides.

Yu Xiang X   Sargaeva Nadezda P NP   Thompson Christopher J CJ   Costello Catherine E CE   Lin Cheng C  

International journal of mass spectrometry 20151101


Deamidation and the subsequent formation of isoaspartic acid (isoAsp) are common modifications of asparagine (Asn) residues in proteins. Differentiation of isoAsp and Asp residues is a challenging task owing to their similar chemical properties and identical molecular mass. Recent studies showed that they can be differentiated using electron capture dissociation (ECD) which generates diagnostic fragments <i>c</i>'+57 and <i>z</i><sup>•</sup>-57 specific to the isoAsp residue. However, the ECD ap  ...[more]

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