Ontology highlight
ABSTRACT:
SUBMITTER: Hense A
PROVIDER: S-EPMC4673609 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Hense Anika A Prunsche Benedikt B Gao Peng P Ishitsuka Yuji Y Nienhaus Karin K Nienhaus G Ulrich GU
Scientific reports 20151209
The advancement of far-red emitting variants of the green fluorescent protein (GFP) is crucially important for imaging live cells, tissues and organisms. Despite notable efforts, far-red marker proteins still need further optimization to match the performance of their green counterparts. Here we present mGarnet, a robust monomeric marker protein with far-red fluorescence peaking at 670 nm. Thanks to its large extinction coefficient of 95,000 M(-1)cm(-1), mGarnet can be efficiently excited with 6 ...[more]