Ontology highlight
ABSTRACT:
SUBMITTER: Machida T
PROVIDER: S-EPMC4673905 | biostudies-literature | 2015 May
REPOSITORIES: biostudies-literature
Machida Takuya T Lang Kathrin K Xue Lin L Chin Jason W JW Winssinger Nicolas N
Bioconjugate chemistry 20150424 5
Efficient access to proteins modified site-specifically with glycans is important in glycobiology and for therapeutic applications. Herein, we report a biocompatible protein glycoconjugation by inverse demand Diels-Alder reaction between tetrazine and trans-cyclooctene. Tetrazine functionalized glycans were obtained in one step by CuAAC (Cu-catalyzed alkyne azide cycloaddition) between glycosyl azide and an alkyne-tetrazine adduct. Site-specific glycoconjugation was performed chemoselectively on ...[more]