Ontology highlight
ABSTRACT:
SUBMITTER: Harris GG
PROVIDER: S-EPMC4674366 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Harris Golda G GG Lombardi Patrick M PM Pemberton Travis A TA Matsui Tsutomu T Weiss Thomas M TM Cole Kathryn E KE Köksal Mustafa M Murphy Frank V FV Vedula L Sangeetha LS Chou Wayne K W WK Cane David E DE Christianson David W DW
Biochemistry 20151124 48
Geosmin synthase from Streptomyces coelicolor (ScGS) catalyzes an unusual, metal-dependent terpenoid cyclization and fragmentation reaction sequence. Two distinct active sites are required for catalysis: the N-terminal domain catalyzes the ionization and cyclization of farnesyl diphosphate to form germacradienol and inorganic pyrophosphate (PPi), and the C-terminal domain catalyzes the protonation, cyclization, and fragmentation of germacradienol to form geosmin and acetone through a retro-Prins ...[more]