Ontology highlight
ABSTRACT:
SUBMITTER: Haniewicz P
PROVIDER: S-EPMC4674563 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Haniewicz Patrycja P Floris Davide D Farci Domenica D Kirkpatrick Joanna J Loi Maria C MC Büchel Claudia C Bochtler Matthias M Piano Dario D
Frontiers in plant science 20151210
Photosystem II (PSII) occurs in different forms and supercomplexes in thylakoid membranes. Using a transplastomic strain of Nicotiana tabacum histidine tagged on the subunit PsbE, we have previously shown that a mild extraction protocol with β-dodecylmaltoside enriches PSII characteristic of lamellae and grana margins. Here, we characterize residual granal PSII that is not extracted by this first solubilization step. Using affinity purification, we demonstrate that this PSII fraction consists of ...[more]