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A genome-wide survey of DNA methylation in hexaploid wheat.

ABSTRACT: DNA methylation is an important mechanism of epigenetic gene expression control that can be passed between generations. Here, we use sodium bisulfite treatment and targeted gene enrichment to study genome-wide methylation across the three sub-genomes of allohexaploid wheat.While the majority of methylation is conserved across all three genomes we demonstrate that differential methylation exists between the sub-genomes in approximately equal proportions. We correlate sub-genome-specific promoter methylation with decreased expression levels and show that altered growing temperature has a small effect on methylation state, identifying a small but functionally relevant set of methylated genes. Finally, we demonstrate long-term methylation maintenance using a comparison between the D sub-genome of hexaploid wheat and its progenitor Aegilops tauschii.We show that tri-genome methylation is highly conserved with the diploid wheat progenitor while sub-genome-specific methylation shows more variation.

SUBMITTER: Gardiner LJ

PROVIDER: S-EPMC4674939 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

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A genome-wide survey of DNA methylation in hexaploid wheat.

Gardiner Laura-Jayne LJ Quinton-Tulloch Mark M Olohan Lisa L Price Jonathan J Hall Neil N Hall Anthony A

Genome biology 20151210

<h4>Background</h4>DNA methylation is an important mechanism of epigenetic gene expression control that can be passed between generations. Here, we use sodium bisulfite treatment and targeted gene enrichment to study genome-wide methylation across the three sub-genomes of allohexaploid wheat.<h4>Results</h4>While the majority of methylation is conserved across all three genomes we demonstrate that differential methylation exists between the sub-genomes in approximately equal proportions. We corr ...[more]

PMID: 26653535

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A genome-wide DNA methylation survey of hexaploid wheat

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