Unknown

Dataset Information

0

Minimal synthetic cells to study integrin-mediated adhesion.


ABSTRACT: To shed light on cell-adhesion-related molecular pathways, synthetic cells offer the unique advantage of a well-controlled model system with reduced molecular complexity. Herein, we show that liposomes with the reconstituted platelet integrin ?IIb ?3 as the adhesion-mediating transmembrane protein are a functional minimal cell model for studying cellular adhesion mechanisms in a defined environment. The interaction of these synthetic cells with various extracellular matrix proteins was analyzed using a quartz crystal microbalance with dissipation monitoring. The data indicated that integrin was functionally incorporated into the lipid vesicles, thus enabling integrin-specific adhesion of the engineered liposomes to fibrinogen- and fibronectin-functionalized surfaces. Then, we were able to initiate the detachment of integrin liposomes from these surfaces in the presence of the peptide GRGDSP, a process that is even faster with our newly synthesized peptide mimetic SN529, which specifically inhibits the integrin ?IIb ?3 .

SUBMITTER: Frohnmayer JP 

PROVIDER: S-EPMC4675076 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3823432 | biostudies-literature
| S-EPMC2064315 | biostudies-literature
| S-EPMC2931747 | biostudies-literature
| S-EPMC1383524 | biostudies-literature
| S-EPMC9030014 | biostudies-literature
| S-EPMC2696803 | biostudies-literature
| S-EPMC10690223 | biostudies-literature
| S-EPMC6995566 | biostudies-literature
| S-EPMC9948217 | biostudies-literature
| S-EPMC2890464 | biostudies-literature