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Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells.

ABSTRACT: We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells.

SUBMITTER: Chung EY

PROVIDER: S-EPMC4675668 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Activatable and Cell-Penetrable Multiplex FRET Nanosensor for Profiling MT1-MMP Activity in Single Cancer Cells.

Chung Eddie Y EY Ochs Christopher J CJ Wang Yi Y Lei Lei L Qin Qin Q Smith Andrew M AM Strongin Alex Y AY Kamm Roger R Qi Ying-Xin YX Lu Shaoying S Wang Yingxiao Y

Nano letters 20150727 8

We developed a quantum-dot-based fluorescence resonance energy transfer (QD-FRET) nanosensor to visualize the activity of matrix metalloproteinase (MT1-MMP) at cell membrane. A bended peptide with multiple motifs was engineered to position the FRET pair at a close proximity to allow energy transfer, which can be cleaved by active MT1-MMP to result in FRET changes and the exposure of cell penetrating sequence. Via FRET and penetrated QD signals, the nanosensor can profile cancer cells. ...[more]

PMID: 26203778

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