Dataset Information

Early Human Papilloma Virus (HPV) Oncogenic Influences in Breast Cancer.

ABSTRACT:

Background

Human papilloma viruses (HPVs) may act early in breast oncogenesis ("hit-and-run" phenomena).

Methods

The authors used immunohistochemistry for the identification of HPV E7 oncogenic protein expression in 32 sets of benign and subsequent breast cancer specimens from the same Australian patients.

Results

HPV E7 oncoprotein was clearly expressed in the nuclei of 23 (72%) of the 32 benign specimens and 20 (62.5%) of the subsequent 32 breast cancer specimens in the same patients. There was no HPV E7 protein expression in seven (30%) of the 23 breast cancer specimens that had prior HPV E7 protein-positive benign breast biopsies in the same patients.

Conclusions

This observation suggests that HPV oncogenic influences occur early in some breast cancers. This finding confirms the previous observations. This early influence of HPVs may be the reason why there is no increase in the prevalence of HPV-associated breast cancer in immunocompromised patients as compared to HPV-associated cervical cancer.

SUBMITTER: Ngan C

PROVIDER: S-EPMC4676430 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Publications

Early Human Papilloma Virus (HPV) Oncogenic Influences in Breast Cancer.

Ngan Christopher C Lawson James S JS Clay Rosemary R Delprado Warick W Whitaker Noel J NJ Glenn Wendy K WK

Breast cancer : basic and clinical research 20151210

<h4>Background</h4>Human papilloma viruses (HPVs) may act early in breast oncogenesis ("hit-and-run" phenomena).<h4>Methods</h4>The authors used immunohistochemistry for the identification of HPV E7 oncogenic protein expression in 32 sets of benign and subsequent breast cancer specimens from the same Australian patients.<h4>Results</h4>HPV E7 oncoprotein was clearly expressed in the nuclei of 23 (72%) of the 32 benign specimens and 20 (62.5%) of the subsequent 32 breast cancer specimens in the s ...[more]

PMID: 26691275

Similar Datasets

Project description:PURPOSE:Human papillomaviruses (HPV) may have a role in some breast cancers. The purpose of this study is to fill important gaps in the evidence. These gaps are: (i) confirmation of the presence of high risk for cancer HPVs in breast cancers, (ii) evidence of HPV infections in benign breast tissues prior to the development of HPV-positive breast cancer in the same patients, (iii) evidence that HPVs are biologically active and not harmless passengers in breast cancer. METHODS:RNA-seq data from The Cancer Genome Atlas (TCGA) was used to identify HPV RNA sequences in breast cancers. We also conducted a retrospective cohort study based on polymerase chain reaction (PCR) analyses to identify HPVs in archival specimens from Australian women with benign breast biopsies who later developed breast cancer. To assess whether HPVs in breast cancer were biologically active,?the expression of the oncogenic protein HPV E7 was assessed by immunohistochemistry (IHC). RESULTS:Thirty (3.5%) low-risk and 20 (2.3%) high-risk HPV types were identified in 855 breast cancers from the TCGA database. The high risk types were HPV 18 (48%), HPV 113 (24%), HPV 16 (10%), HPV 52 (10%). Data from the PCR cohort study indicated that HPV type 18 was the most common type identified in breast cancer specimens (55% of 40 breast cancer specimens) followed by HPV 16 (13%). The same HPV type was identified in both the benign and subsequent breast cancer in 15 patients. HPV E7 proteins were identified in 72% of benign breast specimens and 59% of invasive breast cancer specimens. CONCLUSION:There were four observations of particular interest: (i) confirmation by both NGS and PCR of the presence of high-risk HPV gene sequences in breast cancers, (ii) a correlation between high-risk HPV in benign breast specimens and subsequent HPV-positive breast cancer in the same patient, (iii) HPVs in breast cancer are likely to be biologically active (as shown by transcription of HPV DNA to RNA plus the expression of HPV E7 proteins), (iv) HPV oncogenic influences may occur early in the development of breast cancer.

Dataset Information

Early Human Papilloma Virus (HPV) Oncogenic Influences in Breast Cancer.

Background

Methods

Results

Conclusions

Publications

Early Human Papilloma Virus (HPV) Oncogenic Influences in Breast Cancer.

Similar Datasets

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