Developmental validation of an X-Insertion/Deletion polymorphism panel and application in HAN population of China.

ABSTRACT: InDels are short-length polymorphisms characterized by low mutation rates, high inter-population diversity, short amplicon strategy and simplicity of laboratory analysis. This work describes the developmental validation of an X-InDels panel amplifying 18 bi-allelic markers and Amelogenin in one single PCR system. Developmental validation indicated that this novel panel was reproducible, accurate, sensitive and robust for forensic application. Sensitivity testing of the panel was such that a full profile was obtainable even with 125 pg of human DNA with intra-locus balance above 70%. Specificity testing was demonstrated by the lack of cross-reactivity with a variety of commonly encountered animal species and microorganisms. For the stability testing in cases of PCR inhibition, full profiles

SUBMITTER: Zhang S 

PROVIDER: S-EPMC4677316 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature