Ontology highlight
ABSTRACT:
SUBMITTER: Iwasa T
PROVIDER: S-EPMC4677358 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Iwasa Takuma T Han Yong-Woon YW Hiramatsu Ryo R Yokota Hiroaki H Nakao Kimiko K Yokokawa Ryuji R Ono Teruo T Harada Yoshie Y
Scientific reports 20151214
The Escherichia coli RuvB hexameric ring motor proteins, together with RuvAs, promote branch migration of Holliday junction DNA. Zero mode waveguides (ZMWs) constitute of nanosized holes and enable the visualization of a single fluorescent molecule under micromolar order of the molecules, which is applicable to characterize the formation of RuvA-RuvB-Holliday junction DNA complex. In this study, we used ZMWs and counted the number of RuvBs binding to RuvA-Holliday junction DNA complex. Our data ...[more]