Ontology highlight
ABSTRACT:
SUBMITTER: Wang H
PROVIDER: S-EPMC4677799 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Wang Hong H Wong Chee-Hong CH Chin Alice A Taguchi Ayumu A Taylor Allen A Hanash Samir S Sekiya Sadanori S Takahashi Hidenori H Murase Masaki M Kajihara Shigeki S Iwamoto Shinichi S Tanaka Koichi K
Nature protocols 20110210 3
We present a protocol for the identification of glycosylated proteins in plasma followed by elucidation of their individual glycan compositions. The study of glycoproteins by mass spectrometry is usually based on cleavage of glycans followed by separate analysis of glycans and deglycosylated proteins, which limits the ability to derive glycan compositions for individual glycoproteins. The methodology described here consists of 2D HPLC fractionation of intact proteins and liquid chromatography-mu ...[more]