Ontology highlight
ABSTRACT:
SUBMITTER: Ban K
PROVIDER: S-EPMC4682289 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Ban Kiwon K Wile Brian B Cho Kyu-Won KW Kim Sangsung S Song Ming-Ke MK Kim Sang Yoon SY Singer Jason J Syed Anum A Yu Shan Ping SP Wagner Mary M Bao Gang G Yoon Young-Sup YS
Stem cell reports 20151201 6
Isolation of ventricular cardiomyocytes (vCMs) has been challenging due to the lack of specific surface markers. Here we show that vCMs can be purified from differentiating mouse embryonic stem cells (mESCs) using molecular beacons (MBs) targeting specific intracellular mRNAs. We designed MBs (IRX4 MBs) to target mRNA encoding Iroquois homeobox protein 4 (Irx4), a transcription factor specific for vCMs. To purify mESC vCMs, IRX4 MBs were delivered into cardiomyogenically differentiating mESCs, a ...[more]