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HBD-3 induces NK cell activation, IFN-? secretion and mDC dependent cytolytic function.

ABSTRACT: We previously showed that human beta defensin-3 (hBD-3) activates mDC via TLR1/2. Here we investigated the effects of hBD-3 on NK cell activation state and effector functions. We observed that hBD-3 activates PBMC to secrete IFN-? and kill K562 and HUH hepatoma target cells in an NK dependent fashion, and both TLR1/2 and CCR2 are involved. TLR1, TLR2 and CCR2 were expressed on NK cells, and in purified NK culture experiments we observed hBD-3 to directly act on NK cells, resulting in CD69 upregulation and IFN? secretion. We also observed mDC-hBD-3 enhanced NK cytolytic activity and IFN? production. These results implicate hBD-3 in its ability to directly activate NK cells and increase NK cell effector function, as well as promote mDC-dependent NK activity. HBD-3 may therefore act as a mediator of innate cell interactions that result in bridging of innate and adaptive immunity.

SUBMITTER: Judge CJ

PROVIDER: S-EPMC4682877 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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HBD-3 induces NK cell activation, IFN-γ secretion and mDC dependent cytolytic function.

Judge Chelsey J CJ Reyes-Aviles Elane E Conry Sara J SJ Sieg Scott S SS Feng Zhimin Z Weinberg Aaron A Anthony Donald D DD

Cellular immunology 20150630 2

We previously showed that human beta defensin-3 (hBD-3) activates mDC via TLR1/2. Here we investigated the effects of hBD-3 on NK cell activation state and effector functions. We observed that hBD-3 activates PBMC to secrete IFN-γ and kill K562 and HUH hepatoma target cells in an NK dependent fashion, and both TLR1/2 and CCR2 are involved. TLR1, TLR2 and CCR2 were expressed on NK cells, and in purified NK culture experiments we observed hBD-3 to directly act on NK cells, resulting in CD69 upregu ...[more]

PMID: 26302933

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