Dataset Information

Exposure to 17?-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida.

ABSTRACT: BACKGROUND:The environmental impacts of various substances on all levels of organisms are under investigation. Among these substances, endocrine-disrupting compounds (EDCs) present a threat, although the environmental significance of these compounds remains largely unknown. To shed some light on this field, we assessed the effects of 17?-oestradiol on the growth, reproduction and formation of free radicals in Eisenia fetida. METHODOLOGY/PRINCIPAL FINDINGS:Although the observed effects on growth and survival were relatively weak, a strong impact on reproduction was observed (50.70% inhibition in 100 ?g/kg of E2). We further demonstrated that the exposure of the earthworm Eisenia fetida to a contaminant of emerging concern, 17?-oestradiol (E2), significantly affected the molecules involved in antioxidant defence. Exposure to E2 results in the production of reactive oxygen species (ROS) and the stimulation of antioxidant systems (metallothionein and reduced oxidized glutathione ratio) but not phytochelatins at both the mRNA and translated protein levels. Matrix-assisted laser desorption/ionization (MALDI)-imaging revealed the subcuticular bioaccumulation of oestradiol-3,4-quinone, altering the levels of local antioxidants in a time-dependent manner. CONCLUSIONS/SIGNIFICANCE:The present study illustrates that although most invertebrates do not possess oestrogen receptors, these organisms can be affected by oestrogen hormones, likely reflecting free diffusion into the cellular microenvironment with subsequent degradation to molecules that undergo redox cycling, producing ROS, thereby increasing environmental contamination that also perilously affects keystone animals, forming lower trophic levels.

SUBMITTER: Heger Z

PROVIDER: S-EPMC4690593 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Exposure to 17β-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida.

Heger Zbynek Z Michalek Petr P Guran Roman R Havelkova Barbora B Kominkova Marketa M Cernei Natalia N Richtera Lukas L Beklova Miroslava M Adam Vojtech V Kizek Rene R

PloS one 20151222 12

<h4>Background</h4>The environmental impacts of various substances on all levels of organisms are under investigation. Among these substances, endocrine-disrupting compounds (EDCs) present a threat, although the environmental significance of these compounds remains largely unknown. To shed some light on this field, we assessed the effects of 17β-oestradiol on the growth, reproduction and formation of free radicals in Eisenia fetida.<h4>Methodology/principal findings</h4>Although the observed eff ...[more]

PMID: 26695684

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Project description:Earthworms (Eisenia fetida) were exposed to OECD soils contaminated with arsenite (29.3 mg kg-1), arsenate (35.2 mg kg-1), monomethylarsonate (342.5 mg kg-1) and dimethylarsinate (373.0 mg kg-1) for 64 days. The exposure concentration for the four arsenic species was set at one-tenth of 14 d-LC50 in order to compare their toxicity. Eight biomarkers including superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, reduced glutathione, lipid peroxidation and metallothioneins were analyzed in the organisms. A multi-biomarker approach, the integrated biomarker response (IBR) index, was adopted to summarize the multi-biomarker responses to a single value, reflecting the integrated stress of different arsenic species on earthworms. Furthermore, total arsenic and arsenic speciation were analyzed in earthworm tissue to evaluate the relationship between arsenic accumulation and biomarker responses at the molecular and subcellular levels and to observe the role of arsenic biotransformation in earthworms. The results showed that the toxicity of the four arsenic species was ranked as: arsenite > arsenate > monomethylarsonate and dimethylarsinate. Although organic arsenics showed a low degree of biotoxicity, they could be turned into highly toxic inorganic arsenics under the effect of demethylation, which caused a toxic effect on organisms. The biomarker responses indicated that a sub-lethal dose of both arsenite and arsenate could trigger the response of the antioxidant defense system and cause oxidative damage when the protective capacity of the system was exhausted. Arsenic in earthworms could be detoxified during the process of biotransformation, where inorganic arsenics were converted into organic arsenics, which would then be excreted out. Based on these results, it was proved that different arsenic species showed different degrees of toxicity. Therefore, arsenic species should be differentiated in order to obtain accurate results in quality/risk assessment programs.

Project description:Biochar is the degradation-resistant product generated by the pyrolysis of organic materials and is produced for the intended use of land application in order to promote carbon sequestration and soil improvement. However, despite the many potential benefits biochar application offers, it is important to quantify any ecological impacts that may result from soil amendment in order to avoid potentially causing negative effects upon soil biota which are crucial in the many ecosystem services provided by soil. Any impacts on earthworms in particular are important to evaluate because of their pivotal role in organic matter breakdown, nutrient cycling and soil formation. In this study, we conducted a series of ecotoxicological assays to determine lethal and sublethal (avoidance, mass change and moisture content) effects of heavy biochar applications that reflect levels that may be used in soil restoration efforts. Two earthworm species, Eisenia fetida, an epigeic species, and Lumbricus terrestris, an anecic species, were utilised as test organisms. Two types of biochar, produced from wheat straw and rice husk feedstocks, respectively, were applied to OECD artificial soil and to a natural soil (Kettering loam) at rates of up to 20% w/w. The influence of biochar application on soil porewater chloride, fluoride and phosphate concentrations was also assessed. The biochar applications induced only a subtle level of avoidance behaviour while effects on survival over a 4-week exposure period were inconsistent. However, death and physical damage to some individual earthworms at high biochar application rates were observed, the mechanisms and processes leading to which should be investigated further. Earthworm development (mean mass change over time) proved to be a more sensitive measure, revealing negative effects on L. terrestris at 10% and 20% (w/w) wheat biochar applications in OECD soil and at 20% (w/w) applications of both biochars in Kettering loam. The moisture content of E. fetida remained remarkably consistent across all treatments (~ 82%), indicating that this is not a sensitive measure of effects. The high rates of biochar application resulted in increased chloride (2 to 3-fold) and phosphate (100-fold) concentrations in simulated soil porewaters, which has important implications for soil fertility and production but also for environmental management.

Dataset Information

Exposure to 17?-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida.

Publications

Exposure to 17β-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida.

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