Dataset Information

Exposure to 17?-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida.

ABSTRACT: BACKGROUND:The environmental impacts of various substances on all levels of organisms are under investigation. Among these substances, endocrine-disrupting compounds (EDCs) present a threat, although the environmental significance of these compounds remains largely unknown. To shed some light on this field, we assessed the effects of 17?-oestradiol on the growth, reproduction and formation of free radicals in Eisenia fetida. METHODOLOGY/PRINCIPAL FINDINGS:Although the observed effects on growth and survival were relatively weak, a strong impact on reproduction was observed (50.70% inhibition in 100 ?g/kg of E2). We further demonstrated that the exposure of the earthworm Eisenia fetida to a contaminant of emerging concern, 17?-oestradiol (E2), significantly affected the molecules involved in antioxidant defence. Exposure to E2 results in the production of reactive oxygen species (ROS) and the stimulation of antioxidant systems (metallothionein and reduced oxidized glutathione ratio) but not phytochelatins at both the mRNA and translated protein levels. Matrix-assisted laser desorption/ionization (MALDI)-imaging revealed the subcuticular bioaccumulation of oestradiol-3,4-quinone, altering the levels of local antioxidants in a time-dependent manner. CONCLUSIONS/SIGNIFICANCE:The present study illustrates that although most invertebrates do not possess oestrogen receptors, these organisms can be affected by oestrogen hormones, likely reflecting free diffusion into the cellular microenvironment with subsequent degradation to molecules that undergo redox cycling, producing ROS, thereby increasing environmental contamination that also perilously affects keystone animals, forming lower trophic levels.

SUBMITTER: Heger Z

PROVIDER: S-EPMC4690593 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Exposure to 17β-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida.

Heger Zbynek Z Michalek Petr P Guran Roman R Havelkova Barbora B Kominkova Marketa M Cernei Natalia N Richtera Lukas L Beklova Miroslava M Adam Vojtech V Kizek Rene R

PloS one 20151222 12

<h4>Background</h4>The environmental impacts of various substances on all levels of organisms are under investigation. Among these substances, endocrine-disrupting compounds (EDCs) present a threat, although the environmental significance of these compounds remains largely unknown. To shed some light on this field, we assessed the effects of 17β-oestradiol on the growth, reproduction and formation of free radicals in Eisenia fetida.<h4>Methodology/principal findings</h4>Although the observed eff ...[more]

PMID: 26695684

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Project description:Earthworms (Eisenia fetida) were exposed to OECD soils contaminated with arsenite (29.3 mg kg-1), arsenate (35.2 mg kg-1), monomethylarsonate (342.5 mg kg-1) and dimethylarsinate (373.0 mg kg-1) for 64 days. The exposure concentration for the four arsenic species was set at one-tenth of 14 d-LC50 in order to compare their toxicity. Eight biomarkers including superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase, reduced glutathione, lipid peroxidation and metallothioneins were analyzed in the organisms. A multi-biomarker approach, the integrated biomarker response (IBR) index, was adopted to summarize the multi-biomarker responses to a single value, reflecting the integrated stress of different arsenic species on earthworms. Furthermore, total arsenic and arsenic speciation were analyzed in earthworm tissue to evaluate the relationship between arsenic accumulation and biomarker responses at the molecular and subcellular levels and to observe the role of arsenic biotransformation in earthworms. The results showed that the toxicity of the four arsenic species was ranked as: arsenite > arsenate > monomethylarsonate and dimethylarsinate. Although organic arsenics showed a low degree of biotoxicity, they could be turned into highly toxic inorganic arsenics under the effect of demethylation, which caused a toxic effect on organisms. The biomarker responses indicated that a sub-lethal dose of both arsenite and arsenate could trigger the response of the antioxidant defense system and cause oxidative damage when the protective capacity of the system was exhausted. Arsenic in earthworms could be detoxified during the process of biotransformation, where inorganic arsenics were converted into organic arsenics, which would then be excreted out. Based on these results, it was proved that different arsenic species showed different degrees of toxicity. Therefore, arsenic species should be differentiated in order to obtain accurate results in quality/risk assessment programs.

Dataset Information

Exposure to 17?-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida.

Publications

Exposure to 17β-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida.

Similar Datasets

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