Dataset Information

In vivo mapping of macroscopic neuronal projections in the mouse hippocampus using high-resolution diffusion MRI.

ABSTRACT: Recent developments in diffusion magnetic resonance imaging (MRI) make it a promising tool for non-invasive mapping of the spatial organization of axonal and dendritic networks in gray matter regions of the brain. Given the complex cellular environments, in which these networks reside, evidence on the capability of diffusion MRI-based tractography to study these networks is still lacking. In this study, we used a localized diffusion MRI approach to acquire high spatial and angular resolution images of the live mouse hippocampus. The diffusion MRI and tractography results were compared with histology and the Allen mouse brain connectivity atlas using a multi-step image registration pipeline. The results demonstrated that in vivo diffusion MRI data at 0.1mm isotropic resolution revealed the organization of axonal and dendritic networks in the hippocampus and the tractography results shared remarkable similarity with the viral tracer data in term of their spatial projection patterns. Quantitative analysis showed significant correlations between tractography- and tracer-based projection density measurements in the mouse hippocampus. These findings suggest that high-resolution diffusion MRI and tractography can reveal macroscopic neuronal projections in the mouse hippocampus and are important for future development of advanced tractography methods.

SUBMITTER: Wu D

PROVIDER: S-EPMC4691395 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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In vivo mapping of macroscopic neuronal projections in the mouse hippocampus using high-resolution diffusion MRI.

Wu Dan D Zhang Jiangyang J Zhang Jiangyang J

NeuroImage 20151021

Recent developments in diffusion magnetic resonance imaging (MRI) make it a promising tool for non-invasive mapping of the spatial organization of axonal and dendritic networks in gray matter regions of the brain. Given the complex cellular environments, in which these networks reside, evidence on the capability of diffusion MRI-based tractography to study these networks is still lacking. In this study, we used a localized diffusion MRI approach to acquire high spatial and angular resolution ima ...[more]

PMID: 26499812

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Project description:The hippocampus is classically divided into mesoscopic subfields which contain varying microstructure that contribute to their unique functional roles. It has been challenging to characterize this microstructure with current magnetic resonance based neuroimaging techniques. In this work, we used diffusion magnetic resonance imaging (dMRI) and a novel surface-based approach in the hippocampus which revealed distinct microstructural distributions of neurite density and dispersion, T1w/T2w ratio as a proxy for myelin content, fractional anisotropy, and mean diffusivity. We used the neurite orientation dispersion and density imaging (NODDI) model optimized for grey matter diffusivity to characterize neurite density and dispersion. We found that neurite dispersion was highest in the cornu ammonis (CA) 1 and subiculum subfields which likely captures the large heterogeneity of tangential and radial fibres, such as the Schaffer collaterals, perforant path, and pyramidal neurons. Neurite density and T1w/T2w were highest in the subiculum and CA3 and lowest in CA1, which may reflect known myeloarchitectonic differences between these subfields. Using a simple logistic regression model, we showed that neurite density, dispersion, and T1w/T2w measures were separable across the subfields, suggesting that they may be sensitive to the known variability in subfield cyto- and myeloarchitecture. We report macrostructural measures of gyrification, thickness, and curvature that were in line with ex vivo descriptions of hippocampal anatomy. We employed a multivariate orthogonal projective non-negative matrix factorization (OPNNMF) approach to capture co-varying regions of macro- and microstructure across the hippocampus. The clusters were highly variable along the medial-lateral (proximal-distal) direction, likely reflecting known differences in morphology, cytoarchitectonic profiles, and connectivity. Finally, we show that by examining the main direction of diffusion relative to canonical hippocampal axes, we could identify regions with stereotyped microstructural orientations that may map onto specific fibre pathways, such as the Schaffer collaterals, perforant path, fimbria, and alveus. These results highlight the value of combining in vivo dMRI with computational approaches for capturing hippocampal microstructure, which may provide useful features for understanding cognition and for diagnosis of disease states.

Dataset Information

In vivo mapping of macroscopic neuronal projections in the mouse hippocampus using high-resolution diffusion MRI.

Publications

In vivo mapping of macroscopic neuronal projections in the mouse hippocampus using high-resolution diffusion MRI.

Similar Datasets

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