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Protective Effects of D-Penicillamine on Catecholamine-Induced Myocardial Injury.

ABSTRACT: Iron and copper release participates in the myocardial injury under ischemic conditions and hence protection might be achieved by iron chelators. Data on copper chelation are, however, sparse. The effect of the clinically used copper chelator D-penicillamine in the catecholamine model of acute myocardial injury was tested in cardiomyoblast cell line H9c2 and in Wistar Han rats. D-Penicillamine had a protective effect against catecholamine-induced injury both in vitro and in vivo. It protected H9c2 cells against the catecholamine-induced viability loss in a dose-dependent manner. In animals, both intravenous D-penicillamine doses of 11 (low) and 44?mg/kg (high) decreased the mortality caused by s.c. isoprenaline (100?mg/kg) from 36% to 14% and 22%, respectively. However, whereas the low D-penicillamine dose decreased the release of cardiac troponin T (specific marker of myocardial injury), the high dose resulted in an increase. Interestingly, the high dose led to a marked elevation in plasma vitamin C. This might be related to potentiation of oxidative stress, as suggested by additional in vitro experiments with D-penicillamine (iron reduction and the Fenton reaction). In conclusion, D-penicillamine has protective potential against catecholamine-induced cardiotoxicity; however the optimal dose selection seems to be crucial for further application.

SUBMITTER: Riha M

PROVIDER: S-EPMC4691625 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Protective Effects of D-Penicillamine on Catecholamine-Induced Myocardial Injury.

Říha Michal M Hašková Pavlína P Martin Jan J Filipský Tomáš T Váňová Kateřina K Vávrová Jaroslava J Holečková Magdalena M Homola Pavel P Vítek Libor L Palicka Vladimír V Šimůnek Tomáš T Mladěnka Přemysl P

Oxidative medicine and cellular longevity 20151214

Iron and copper release participates in the myocardial injury under ischemic conditions and hence protection might be achieved by iron chelators. Data on copper chelation are, however, sparse. The effect of the clinically used copper chelator D-penicillamine in the catecholamine model of acute myocardial injury was tested in cardiomyoblast cell line H9c2 and in Wistar Han rats. D-Penicillamine had a protective effect against catecholamine-induced injury both in vitro and in vivo. It protected H9 ...[more]

PMID: 26788248

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Protective Effects of D-Penicillamine on Catecholamine-Induced Myocardial Injury.

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Protective Effects of D-Penicillamine on Catecholamine-Induced Myocardial Injury.

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