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Polymorphism of flagellin A gene in Helicobacter pylori.


ABSTRACT: AIM:To study the polymorphism of flagellin A genotype and its significance in Helicobacter pylori (H. pylori). METHODS:As the template, genome DNA was purified from six clinical isolates of H. pylori from outpatients, and the corresponding flagellin A fragments were amplified by polymerase chain reaction. All these products were sequenced. These sequences were compared with each other, and analyzed by software of FASTA program. RESULTS:Specific PCR products were amplified from all of these H. pylori isolates and no length divergence was found among them. Compared with each other, the highest ungapped identity is 99.10%, while the lowest is 94.65%. Using FASTA program, the alignments between query and library sequences derived from different H. pylori strains were higher than 90%. CONCLUSION:The nucleotide sequence of flagellin A in H. pylori is highly conservative with incident divergence. This information may be useful for gene diagnosis and further study on flagellar antigen phenotype.

SUBMITTER: Ji WS 

PROVIDER: S-EPMC4695594 | biostudies-literature | 2001 Dec

REPOSITORIES: biostudies-literature

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Polymorphism of flagellin A gene in Helicobacter pylori.

Ji W S WS   Hu J L JL   Qiu J W JW   Peng D R DR   Shi B L BL   Zhou S J SJ   Wu K C KC   Fan D M DM  

World journal of gastroenterology 20011201 6


<h4>Aim</h4>To study the polymorphism of flagellin A genotype and its significance in Helicobacter pylori (H. pylori).<h4>Methods</h4>As the template, genome DNA was purified from six clinical isolates of H. pylori from outpatients, and the corresponding flagellin A fragments were amplified by polymerase chain reaction. All these products were sequenced. These sequences were compared with each other, and analyzed by software of FASTA program.<h4>Results</h4>Specific PCR products were amplified f  ...[more]

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