De Novo Assembly and Transcriptome Characterization of Canine Retina Using High-Throughput Sequencing.
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ABSTRACT:
SUBMITTER: Reddy B
PROVIDER: S-EPMC4695645 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
De Novo Assembly and Transcriptome Characterization of Canine Retina Using High-Throughput Sequencing.
Reddy Bhaskar B Patel Amrutlal K AK Singh Krishna M KM Patil Deepak B DB Parikh Pinesh V PV Kelawala Divyesh N DN Koringa Prakash G PG Bhatt Vaibhav D VD Rao Mandava V MV Joshi Chaitanya G CG
Genetics research international 20151216
We performed transcriptome sequencing of canine retinal tissue by 454 GS-FLX and Ion Torrent PGM platforms. RNA-Seq analysis by CLC Genomics Workbench mapped expression of 10,360 genes. Gene ontology analysis of retinal transcriptome revealed abundance of transcripts known to be involved in vision associated processes. The de novo assembly of the sequences using CAP3 generated 29,683 contigs with mean length of 560.9 and N50 of 619 bases. Further analysis of contigs predicted 3,827 full-length c ...[more]
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