Ontology highlight
ABSTRACT:
SUBMITTER: Lelyveld VS
PROVIDER: S-EPMC4697197 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Lelyveld Victor S VS Björkbom Anders A Ransey Elizabeth M EM Sliz Piotr P Szostak Jack W JW
Journal of the American Chemical Society 20151203 49
High affinity RNA-protein interactions are critical to cellular function, but directly identifying the determinants of binding within these complexes is often difficult. Here, we introduce a stable isotope mass labeling technique to assign specific interacting nucleotides in an oligonucleotide-protein complex by photo-cross-linking. The method relies on generating site-specific oxygen-18-labeled phosphodiester linkages in oligonucleotides, such that covalent peptide-oligonucleotide cross-link si ...[more]