The Overexpression of TGF-? and CCN2 in Intrauterine Adhesions Involves the NF-?B Signaling Pathway.

ABSTRACT: Intrauterine adhesions (IUA) are a significant cause of menstrual disturbance and infertility, but their pathogenesis still remains unclear. Here, we investigated the expression of TGF-? and CCN2 in IUA endometrial tissue by immunohistochemistry, western blotting and qRT-PCR assays, and found the expression of TGF-? and CCN2 in the endometrial tissue of IUA was significantly increased compared to normal endometrium and uterine septum (P<0.01), suggesting that TGF-? and CCN2 may play an important role in the formation of IUA. Moreover, the activity of the NF-?B signaling pathway in endometrial tissue of IUA was also significantly enhanced compared to normal endometrial and uterine septum (P<0.01) and positively correlated with the expression of TGF-? and CCN2, which suggested that TGF-? and CCN2 expression may be involved in the NF-?B signaling pathway. Blocking the NF-?B signaling pathway using SN50 resulted in the reduced expression of TGF-? in RL95-2 cells, which confirmed the association of the NF-?B signaling pathway and TGF-? in endometrial cells. Additionally, the expression of TGF-? and CCN2 was associated with IUA recurrence, which provides a potential prognostic indictor for IUA. Together, these results demonstrated that TGF-? and CCN2 play an important role in IUA formation, whose mechanism was associated with the activation of the NF-?B signaling pathway.

SUBMITTER: Xue X 

PROVIDER: S-EPMC4697802 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature