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Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles.

ABSTRACT: We describe a system for rapidly screening hundreds of nanoparticle samples using transmission electron microscopy (TEM). The system uses a liquid handling robot to place up to 96 individual samples onto a single standard TEM grid at separate locations. The grid is then transferred into the TEM and automated software is used to acquire multiscale images of each sample. The images are then analyzed to extract metrics on the size, shape, and morphology of the nanoparticles. The system has been used to characterize plasmonically active nanomaterials.

SUBMITTER: Mulligan SK

PROVIDER: S-EPMC4701052 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles.

Mulligan Sean K SK Speir Jeffrey A JA Razinkov Ivan I Cheng Anchi A Crum John J Jain Tilak T Duggan Erika E Liu Er E Nolan John P JP Carragher Bridget B Potter Clinton S CS

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada 20150801 4

We describe a system for rapidly screening hundreds of nanoparticle samples using transmission electron microscopy (TEM). The system uses a liquid handling robot to place up to 96 individual samples onto a single standard TEM grid at separate locations. The grid is then transferred into the TEM and automated software is used to acquire multiscale images of each sample. The images are then analyzed to extract metrics on the size, shape, and morphology of the nanoparticles. The system has been use ...[more]

PMID: 26223550

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