In vivo Calcium Imaging of Evoked Calcium Waves in the Embryonic Cortex.

ABSTRACT: The dynamics of intracellular calcium fluxes are instrumental in the proliferation, differentiation, and migration of neuronal cells. Knowledge thus far of the relationship between these calcium changes and physiological processes in the developing brain has derived principally from ex vivo and in vitro experiments. Here, we present a new method to image intracellular calcium flux in the cerebral cortex of live rodent embryos, whilst attached to the dam through the umbilical cord. Using this approach we demonstrate induction of calcium waves by laser stimulation. These waves are sensitive to ATP-receptor blockade and are significantly increased by pharmacological facilitation of intracellular-calcium release. This approach is the closest to physiological conditions yet achieved for imaging

SUBMITTER: Yuryev M 

PROVIDER: S-EPMC4701926 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature