Ontology highlight
ABSTRACT:
SUBMITTER: El Khatib M
PROVIDER: S-EPMC4702087 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
El Khatib Mariam M Martins Alexandre A Bourgeois Dominique D Colletier Jacques-Philippe JP Adam Virgile V
Scientific reports 20160106
Phototransformable fluorescent proteins are central to several nanoscopy approaches. As yet however, there is no available variant allowing super-resolution imaging in cell compartments that maintain oxidative conditions. Here, we report the rational design of two reversibly switchable fluorescent proteins able to fold and photoswitch in the bacterial periplasm, rsFolder and rsFolder2. rsFolder was designed by hybridisation of Superfolder-GFP with rsEGFP2, and inherited the fast folding properti ...[more]