Dataset Information

Metabolic Profiling and Physiological Analysis of a Novel Rice Introgression Line with Broad Leaf Size.

ABSTRACT: A rice introgression line, NIL-SS1, and its recurrent parent, Teqing, were used to investigate the influence of the introgression segment on plant growth. The current research showed NIL-SS1 had an increased flag leaf width, total leaf area, spikelet number per panicle and grain yield, but a decreased photosynthetic rate. The metabolite differences in NIL-SS1 and Teqing at different developmental stages were assessed using gas chromatography-mass spectrometry technology. Significant metabolite differences were observed across the different stages. NIL-SS1 increased the plant leaf nitrogen content, and the greatest differences between NIL-SS1 and Teqing occurred at the booting stage. Compared to Teqing, the metabolic phenotype of NIL-SS1 at the booting stage has closer association with those at the flowering stage. The introgression segment induced more active competition for sugars and organic acids (OAs) from leaves to the growing young spikes, which resulted in more spikelet number per plant (SNP). The results indicated the introgression segment could improve rice grain yield by increasing the SNP and total leaf area per plant, which resulted from the higher plant nitrogen content across growth stages and stronger competition for sugars and OAs of young spikes at the booting stage.

SUBMITTER: Zhao X

PROVIDER: S-EPMC4703127 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Metabolic Profiling and Physiological Analysis of a Novel Rice Introgression Line with Broad Leaf Size.

Zhao Xiuqin X Zhang Guilian G Wang Yun Y Zhang Fan F Wang Wensheng W Zhang Wenhao W Fu Binying B Xu Jianlong J Li Zhikang Z

PloS one 20151229 12

A rice introgression line, NIL-SS1, and its recurrent parent, Teqing, were used to investigate the influence of the introgression segment on plant growth. The current research showed NIL-SS1 had an increased flag leaf width, total leaf area, spikelet number per panicle and grain yield, but a decreased photosynthetic rate. The metabolite differences in NIL-SS1 and Teqing at different developmental stages were assessed using gas chromatography-mass spectrometry technology. Significant metabolite d ...[more]

PMID: 26713754

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Project description:Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is a devastating rice disease worldwide. Xa39 is a resistance (R) gene with a broad-spectrum hypersensitive response (BSHR) to Xoo. Nevertheless, the molecular mechanisms of resistance mediated by Xa39 remain unclear. In this study, the transcriptome profiling of a rice line carrying Xa39 and its parents at the early stage of Xoo infection were investigated.A rice introgression line H471 carrying Xa39 exhibited a typical local hypersensitive response phenotype, accompanied by programmed cell death after inoculation with the Xoo Philippines' race 9b. Transcriptome profiling of H471 and its parents at 1 and 2 days post-inoculation was performed using RNA sequencing. In total, 306 differentially expressed genes (DEGs) were identified in H471 compared with its recurrent parent Huang-Hua-Zhan after inoculation with Xoo. Among them, 121 (39.5%) genes, with functional enrichments that were related to defense response, protein amino acid phosphorylation, and apoptosis, were found to be constitutively expressed. The other 185 (60.5%) genes, with GO terms that belonged to defense response, were significantly responsive to Xoo infection in H471. Ten up-regulated and 12 down-regulated genes encoding intracellular immune receptors were identified in H471 compared with Huang-Hua-Zhan. LOC_Os11g37759, which was located in the fine-mapping region harboring Xa39, is a Xa39 candidate gene. The putative BSHR-related co-regulatory networks were constructed using 33 DEGs from four functional groups, including gibberellic acid receptors and brassinosteroid regulators, which were differentially co-expressed with LOC_Os11g37759 in infected H471. Our results indicated that there might be cross-talk between the Xa39-mediated signal transduction cascades and the GA/BR signaling pathway, and that the defense mechanism was related to diverse kinases, transcription factors, post-translational regulation, and R genes.The present study provides the comprehensive transcriptome profile of a rice introgression line carrying Xa39 and its parents, and identifies a set of DEGs involved in BSHR mediated by Xa39. These data provide novel insights into the regulatory networks of plant disease resistance mediated by R genes, and the identified DEGs will serve as candidates for Xa39 cloning and for further understanding the molecular mechanism of BSHR.

Project description:BackgroundHybridization between genetically diverged organisms is known as an important avenue that drives plant genome evolution. The possible outcomes of hybridization would be the occurrences of genetic instabilities in the resultant hybrids. It remained under-investigated however whether pollination by alien pollens of a closely related but sexually "incompatible" species could evoke genomic changes and to what extent it may result in phenotypic novelties in the derived progenies.Methodology/principal findingsIn this study, we have re-sequenced the genomes of Oryza sativa ssp. japonica cv. Matsumae and one of its derived introgressant RZ35 that was obtained from an introgressive hybridization between Matsumae and Zizanialatifolia Griseb. in general, 131 millions 90 base pair (bp) paired-end reads were generated which covered 13.2 and 21.9 folds of the Matsumae and RZ35 genomes, respectively. Relative to Matsumae, a total of 41,724 homozygous single nucleotide polymorphisms (SNPs) and 17,839 homozygous insertions/deletions (indels) were identified in RZ35, of which 3,797 SNPs were nonsynonymous mutations. Furthermore, rampant mobilization of transposable elements (TEs) was found in the RZ35 genome. The results of pathogen inoculation revealed that RZ35 exhibited enhanced resistance to blast relative to Matsumae. Notably, one nonsynonymous mutation was found in the known blast resistance gene Pid3/Pi25 and real-time quantitative (q) RT-PCR analysis revealed constitutive up-regulation of its expression, suggesting both altered function and expression of Pid3/Pi25 may be responsible for the enhanced resistance to rice blast by RZ35.Conclusions/significanceOur results demonstrate that introgressive hybridization by Zizania has provoked genomewide, extensive genomic changes in the rice genome, and some of which have resulted in important phenotypic novelties. These findings suggest that introgressive hybridization by alien pollens of even a sexually incompatible species may represent a potent means to generate novel genetic diversities, and which may have played relevant roles in plant evolution and can be manipulated for crop improvements.

Dataset Information

Metabolic Profiling and Physiological Analysis of a Novel Rice Introgression Line with Broad Leaf Size.

Publications

Metabolic Profiling and Physiological Analysis of a Novel Rice Introgression Line with Broad Leaf Size.

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